Difference between revisions of "Part:BBa K1354001:Design"
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<partinfo>BBa_K1354001 short</partinfo> | <partinfo>BBa_K1354001 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | The primers for each step were designed and tested. | ||
===Source=== | ===Source=== | ||
| − | + | TRP1 was contained in pFA6a. Using the protocol primer, the knockout cassette was amplified then used in the transformation of yeast to knock out VPS75. This BioBrick part was taken from the transformed yeast with the VPS75 gene knocked out. | |
===References=== | ===References=== | ||
Latest revision as of 00:02, 10 October 2014
VPS75 gene deletion cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 339
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 102
Illegal BamHI site found at 75 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 339
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 339
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The primers for each step were designed and tested.
Source
TRP1 was contained in pFA6a. Using the protocol primer, the knockout cassette was amplified then used in the transformation of yeast to knock out VPS75. This BioBrick part was taken from the transformed yeast with the VPS75 gene knocked out.