Difference between revisions of "Part:BBa K1354001"
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<partinfo>BBa_K1354001 short</partinfo> | <partinfo>BBa_K1354001 short</partinfo> | ||
| − | + | This sequence is used in Saccharomyces cerevisiae for the knockout of the VPS75 gene with a TRP1 selectable marker. It shortens the initial length of telomeres. In conjunction with an EST2 knockout, the senescence curve of the yeast cells can be compared with those with a single EST2 knockout. | |
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Latest revision as of 00:01, 10 October 2014
VPS75 gene deletion cassette
This sequence is used in Saccharomyces cerevisiae for the knockout of the VPS75 gene with a TRP1 selectable marker. It shortens the initial length of telomeres. In conjunction with an EST2 knockout, the senescence curve of the yeast cells can be compared with those with a single EST2 knockout.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 339
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 102
Illegal BamHI site found at 75 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 339
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 339
- 1000COMPATIBLE WITH RFC[1000]