Difference between revisions of "Part:BBa K1372000"

Latest revision as of 02:10, 18 October 2014

salicylate inducible suppressing system

This Biobrick is the result of the assembly of two Biobrick:

- BBa_J61051 for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).

- BBa_K228001: for the transcription of SupD-tRNA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1312

Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1372000 short</partinfo>
-assembly of the BBa_J61051 biobrick (PSal/NahR biosensor) with the BBa_K228001 biobrick (SupD-tRNA). This construction is an intermediate in the BBa_K1372001 construction
+This Biobrick is the result of the assembly of two Biobrick:
+- [https://parts.igem.org/Part:BBa_J61051 BBa_J61051] for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
+- [https://parts.igem.org/Part:BBa_K228001 BBa_K228001]: for the transcription of SupD-tRNA.
+[[File:Paris Saclay BB2000.png|center]]
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 22: @@
 <partinfo>BBa_K1372000 parameters</partinfo>
 <!-- -->
+Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]