Difference between revisions of "Part:BBa K1439002"
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<partinfo>BBa_K1439002 short</partinfo> | <partinfo>BBa_K1439002 short</partinfo> | ||
| − | + | Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains. | |
Latest revision as of 08:34, 2 October 2014
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1160
Illegal AgeI site found at 1272 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19