Difference between revisions of "Part:BBa K1453000:Design"

Latest revision as of 15:11, 17 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1158

More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.

More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.

BBa_K771000

BBa_E1010

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1453000 short</partinfo>
@@ Line 7: / Line 6: @@
 ===Design Notes===
-When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.
-With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning)
-More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai.
-More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg.
+More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.
+More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.
 ===Source===
 BBa_K771000
 BBa_E1010
 ===References===