Difference between revisions of "Part:BBa K1439001"
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<partinfo>BBa_K1439001 short</partinfo> | <partinfo>BBa_K1439001 short</partinfo> | ||
Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains. | Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains. | ||
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<partinfo>BBa_K1439001 parameters</partinfo> | <partinfo>BBa_K1439001 parameters</partinfo> | ||
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Latest revision as of 02:21, 15 October 2014
This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.
Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 20
Illegal NgoMIV site found at 30
Illegal AgeI site found at 1139
Illegal AgeI site found at 1251 - 1000COMPATIBLE WITH RFC[1000]