Difference between revisions of "Part:BBa K1368008:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | to | + | Firstly, we divided this circuit into two parts: riboswitch and Cre protein with double terminators. When it came to the synthesis of riboswitch, we designed four specific primers which had almost 20 overlapping sequence and conducted twice PCR to get our riboswitch (more details are shown in our protocol). Then, we connected both of them by the standard method of biobrick connection. |
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===Source=== | ===Source=== | ||
− | + | All of the basic parts come from iGEM distribution. | |
===References=== | ===References=== |
Latest revision as of 06:40, 12 October 2014
mcherry generator driven by HSL under the post transcriptional control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 61
Illegal BsaI.rc site found at 300
Design Notes
Firstly, we divided this circuit into two parts: riboswitch and Cre protein with double terminators. When it came to the synthesis of riboswitch, we designed four specific primers which had almost 20 overlapping sequence and conducted twice PCR to get our riboswitch (more details are shown in our protocol). Then, we connected both of them by the standard method of biobrick connection.
Source
All of the basic parts come from iGEM distribution.