Difference between revisions of "Part:BBa K1555000:Design"

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===Design Notes===
 
===Design Notes===
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For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of ''copa'' sequence as our candidates.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.
+
For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of ''copa'' promoter as our candidate.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.
  
 
===Source===
 
===Source===
  
−
We attained the part of''copa'' from E.coli's genome.
+
We attained the part of ''copa'' from E.coli's genome.
  
 
===References===
 
===References===
 
Outten, F. W., Outten, C. E., Hale, J., & O'Halloran, T. V. (2000). Transcriptional Activation of an ''Escherichia coli'' Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR. ''Journal of Biological Chemistry'',275(40), 31024-31029.
 
Outten, F. W., Outten, C. E., Hale, J., & O'Halloran, T. V. (2000). Transcriptional Activation of an ''Escherichia coli'' Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR. ''Journal of Biological Chemistry'',275(40), 31024-31029.

Latest revision as of 19:21, 28 September 2014


The copa promoter wth a gfp reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914


Design Notes

For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of copa promoter as our candidate.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.

Source

We attained the part of copa from E.coli's genome.

References

Outten, F. W., Outten, C. E., Hale, J., & O'Halloran, T. V. (2000). Transcriptional Activation of an Escherichia coli Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR. Journal of Biological Chemistry,275(40), 31024-31029.