Difference between revisions of "Part:BBa K1424003:Design"
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===References=== | ===References=== | ||
+ | Poteete et al. (2006) Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. BioTechniques 41:261-264 | ||
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+ | Guldener U et al (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research, Vol. 24, No. 13 2519–2524. | ||
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+ | Gueldner U et al(2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research, Vol. 30, No. 6 e23. |
Latest revision as of 09:37, 8 October 2014
Kanamycin resistance for Synechocystis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 135
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
genomic DNA of kanamycin containing strain of Synechocystis sp. PCC 6803
References
Poteete et al. (2006) Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. BioTechniques 41:261-264
Guldener U et al (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research, Vol. 24, No. 13 2519–2524.
Gueldner U et al(2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research, Vol. 30, No. 6 e23.