Difference between revisions of "Part:BBa K1330000:Design"
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<partinfo>BBa_K1330000 short</partinfo> | <partinfo>BBa_K1330000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence was synthesised, and amplified using High-Fidelity PCR. | |
| − | + | The PCR product was cut and ligated with pSB1C3. | |
| + | The fusion with tRNA was designed as described in [http://www.ncbi.nlm.nih.gov/pubmed/17558412 Ponchon & Dardel]. | ||
| + | The Spinach2 sequence comes from [http://www.nature.com/nmeth/journal/v10/n12/full/nmeth.2701.html Strack ''et al.'']. This BioBrick is a modified version of Spinach2 that has been modified to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. See [http://2014.igem.org/Team:DTU-Denmark/Achievements/Parts our wiki] for more details. | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Latest revision as of 22:57, 30 October 2014
Spinach2.1 flanked by tRNALys3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was synthesised, and amplified using High-Fidelity PCR. The PCR product was cut and ligated with pSB1C3.
The fusion with tRNA was designed as described in [http://www.ncbi.nlm.nih.gov/pubmed/17558412 Ponchon & Dardel]. The Spinach2 sequence comes from [http://www.nature.com/nmeth/journal/v10/n12/full/nmeth.2701.html Strack et al.]. This BioBrick is a modified version of Spinach2 that has been modified to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. See [http://2014.igem.org/Team:DTU-Denmark/Achievements/Parts our wiki] for more details.