Difference between revisions of "Part:BBa K1486008:Design"
Darth Vader (Talk | contribs) (→Source) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP | + | It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP. |
| + | WARNING: It is also to be noted that we had to submit basic parts with a start codon and a stop codon, which may have been removed to make the composites part, so that only the first protein cds have a START codon and only the last protein cds have a STOP codon. You should consider it when looking at the part sequence. | ||
===Source=== | ===Source=== | ||
| − | + | We received Michnick lab IFP1 and IFP2 sequences from [https://www.addgene.org/Stephen_Michnick/ addgene]. | |
===References=== | ===References=== | ||
| − | Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. <i>Nature Methods</i>, 6-6. | + | Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] <i>Nature Methods</i>, 6-6. |
Latest revision as of 14:20, 17 October 2014
CxpR & Split IFP1.4 [Cterm + Cterm][1]
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3657
Illegal BamHI site found at 1144
Illegal XhoI site found at 1283
Illegal XhoI site found at 2470 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2213
Illegal PstI site found at 3446
Illegal AgeI site found at 979
Illegal AgeI site found at 1694
Illegal AgeI site found at 2881 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP. WARNING: It is also to be noted that we had to submit basic parts with a start codon and a stop codon, which may have been removed to make the composites part, so that only the first protein cds have a START codon and only the last protein cds have a STOP codon. You should consider it when looking at the part sequence.
Source
We received Michnick lab IFP1 and IFP2 sequences from addgene.
References
Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.