Difference between revisions of "Part:BBa K1365000:Design"

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<partinfo>BBa_K1365000 short</partinfo>
 
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===Source===
 
===Source===
  
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Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.
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Obtained by PCR on the genome of ''Lactococcus lactis'' NZ9700, a nisin producing strain.
  
 
===References===
 
===References===
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 +
Trmčić, A. et al. (2011) Complete nisin A gene cluster from ''Lactococcus lactis'' M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. ''Genes Genom.'' 33: 217-221

Latest revision as of 01:43, 18 October 2014

NisA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
to PCR NisA from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

References

Trmčić, A. et al. (2011) Complete nisin A gene cluster from Lactococcus lactis M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. Genes Genom. 33: 217-221