Difference between revisions of "Part:BBa K1379003:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1379003 short</partinfo>
 
<partinfo>BBa_K1379003 short</partinfo>
  
 
<partinfo>BBa_K1379003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1379003 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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None
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===Source===
 
===Source===
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- P<sub>comFA</sub> primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute.<br>
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The promoter is found genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT is BBa_E0240
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- iGEM 2014 Hong_Kong_HKUST Team has cloned P<sub>comFA</sub> from S. pneumoniae strain NCTC7465 by PCR, followed by restriction-ligation with [[Part:BBa_E0240|BBa_E0240]] in [[Part:pSB1C3|pSB1C3]] backbone.
  
 
===References===
 
===References===

Latest revision as of 04:52, 12 October 2014

PcomFA-E0240 (Measurement Kit for PcomFA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 831

Design Notes

None

Source

- PcomFA primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- iGEM 2014 Hong_Kong_HKUST Team has cloned PcomFA from S. pneumoniae strain NCTC7465 by PCR, followed by restriction-ligation with BBa_E0240 in pSB1C3 backbone.

References