Difference between revisions of "Part:BBa K431008:Experience"
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<I>UCSF-UCB iGEM 2014</I>
 
<I>UCSF-UCB iGEM 2014</I>
 
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[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
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We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter.
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We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.
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[[File:140630_pTEF1_GFP_ROUND_1.png|800px]]
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The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.
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pTEF1 is also listed as parts BBa_K563004 and BBa_K563004.
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Latest revision as of 18:25, 8 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K431008

User Reviews

UNIQ6b492f096f9b7e27-partinfo-00000000-QINU


UNIQ6b492f096f9b7e27-partinfo-00000001-QINU

••••

UCSF-UCB iGEM 2014

We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.

140630 pTEF1 GFP ROUND 1.png

The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.

pTEF1 is also listed as parts BBa_K563004 and BBa_K563004.

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