Difference between revisions of "Part:BBa K1520020"

Latest revision as of 01:24, 18 October 2014

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter

It produces luxR-AHL complex when there is enough IPTG.

From left to right, Marker(1Kb), Pcons2-rbs-lacI-T-Plac-rbs-luxI-T, Pcons2-rbs-lacI-T-Plac-rbs-luxI-T-Pcons2-rbs-luxR-Ter. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2380
Illegal NheI site found at 2403
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1208
Illegal BglII site found at 2222
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1520020 short</partinfo>
-Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
+Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
-It produces luxI when there is enough IPTG.
+It produces luxR-AHL complex when there is enough IPTG.
+[[File:k1520020.jpg]]
+From left to right, Marker(1Kb), Pcons2-rbs-lacI-T-Plac-rbs-luxI-T, Pcons2-rbs-lacI-T-Plac-rbs-luxI-T-Pcons2-rbs-luxR-Ter.
+The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
 <!-- Add more about the biology of this part here