Difference between revisions of "Part:BBa K1520017"

Latest revision as of 00:52, 18 October 2014

Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter

Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.

It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp.

From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter

The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2215
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 985

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1520017 short</partinfo>
 Plasmid1 in detector:
-Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
+Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.
-It is a high level detection plasmid. It produces rfp when AHL is not so much.
+It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp.
+[[File:withoutAHL.jpg]]
+[[File:k1520017.jpg]]
+From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
+The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
 <!-- Add more about the biology of this part here