Difference between revisions of "Part:BBa K1520013"
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<partinfo>BBa_K1520013 short</partinfo> | <partinfo>BBa_K1520013 short</partinfo> | ||
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It produces rfp, luxI and luxR when there is enough luxR and AHL. | It produces rfp, luxI and luxR when there is enough luxR and AHL. | ||
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| + | [[File:k1520010.jpg]] | ||
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| + | From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. | ||
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| + | The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | ||
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Latest revision as of 01:18, 18 October 2014
Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.
From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1458
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]