Difference between revisions of "Part:BBa K1520000"

 
 
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<partinfo>BBa_K1520000 short</partinfo>
 
<partinfo>BBa_K1520000 short</partinfo>
  
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MlrD.
 
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The protein is involved in microcystin degradation
 
  
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mrlD is a gene involved in degradation of microsystin. The MlrD protein belongs to a protein family which is predicted to carry small peptides across the bacterial cell wall and it is therefore possible that this protein facilitates the transport of microcystin. The taking in process would facility another protein,MrlA,which is an enzyme of microcystin to clean up microcystin successfully.
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[[File:MCHPLC.jpg]]
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From the HPLC result, we can conclude that express MlrD in bacteria can increase their MC absorbing ability. Although the concentrations of microcystin in two treatments at 0h are different, the decrease of MC in the first hour is faster in MlrD bacteria than it in the control (bacteria without MlrD). The difference at 0h can be explained by the fact that IPTG can kill some of the bacteria, which makes the number of bacteria in the IPTG (+) tube is less than the number in the control tube, and that can result in the absorbing quantity of MlrD bacteria lower than control one at the beginning (So the concentration of MC in this tube is higher at 0h). This fact can also explain the phenomenon that there is a decrease of MC concentration in the control group during the one hour cultivation.
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[[File:k1520000.jpg]]
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From left to right, Marker(DL10000), MlrD.
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Primers used in PCR are VF2 and VR
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 02:00, 18 October 2014

MlrD.The protein is involved in microcystin degradation.


mrlD is a gene involved in degradation of microsystin. The MlrD protein belongs to a protein family which is predicted to carry small peptides across the bacterial cell wall and it is therefore possible that this protein facilitates the transport of microcystin. The taking in process would facility another protein,MrlA,which is an enzyme of microcystin to clean up microcystin successfully.

MCHPLC.jpg

From the HPLC result, we can conclude that express MlrD in bacteria can increase their MC absorbing ability. Although the concentrations of microcystin in two treatments at 0h are different, the decrease of MC in the first hour is faster in MlrD bacteria than it in the control (bacteria without MlrD). The difference at 0h can be explained by the fact that IPTG can kill some of the bacteria, which makes the number of bacteria in the IPTG (+) tube is less than the number in the control tube, and that can result in the absorbing quantity of MlrD bacteria lower than control one at the beginning (So the concentration of MC in this tube is higher at 0h). This fact can also explain the phenomenon that there is a decrease of MC concentration in the control group during the one hour cultivation.

K1520000.jpg

From left to right, Marker(DL10000), MlrD.

Primers used in PCR are VF2 and VR

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1042