Difference between revisions of "Part:BBa K1491020"

 
 
(3 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1491020 short</partinfo>
 
<partinfo>BBa_K1491020 short</partinfo>
  
−
Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).
+
<p>Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).</p>
 +
 
 +
<p>It has been optimized for E. coli by eliminating the rare codons.</p>
 +
<p><b>Characterization:</b></p>
 +
The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)
 +
 
 +
<center>https://static.igem.org/mediawiki/2014/thumb/d/dc/ConstitutiveSuperoxides.jpg/386px-ConstitutiveSuperoxides.jpg</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:53, 18 October 2014

Constitutive Codon Optimized Killer Red

Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).

It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

386px-ConstitutiveSuperoxides.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418