Difference between revisions of "Part:BBa K1351018"

Latest revision as of 16:14, 30 October 2014

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI:	GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI:	TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in Bacillus subtilis. The overhangs were designed according to RFC10.
Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg
Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K1351018 short</partinfo>
-Biobrick flanked Promoter sequence. The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in bacillus subtilis.
+This part was generated with the RFC10 standard and has the following prefix and suffix:
-Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg <br>
+{|
+|prefix with EcoRI, NotI and XbaI:
+|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
+|-
+|suffix with SpeI, NotI and PstI:
+|T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>
+|}
+Sites of restriction enzymes generating compatible overhangs have the same color: <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red.
+This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
+The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in ''Bacillus subtilis''. The overhangs were designed according to RFC10.
+<br>Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg <br>
 Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg