Difference between revisions of "Part:BBa K1351018"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1351018 short</partinfo>
 
  
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Biobrick flanked Promoter sequence. The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in bacillus subtilis.  
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This part was generated with the RFC10 standard and has the following prefix and suffix:
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{|
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|prefix with EcoRI, NotI and XbaI:
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|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
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|-
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|suffix with SpeI, NotI and PstI:
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|T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>
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|}
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Sites of restriction enzymes generating compatible overhangs have the same color: <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red.
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
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The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in ''Bacillus subtilis''. The overhangs were designed according to RFC10.
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<br>Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg <br>
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Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:14, 30 October 2014


This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in Bacillus subtilis. The overhangs were designed according to RFC10.
Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg
Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]