Difference between revisions of "Part:BBa K1499500:Design"
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===Design Notes===
 
===Design Notes===
  
−
Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells.  
+
Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells.
−
 
+
  
 
===Source===
 
===Source===
  
−
Combination of two previous parts: (BBa_I13202 and BBa_T9002)
+
Combination of two previous parts: BBa_I13202 [1] and BBa_T9002 [2].
  
 
===References===
 
===References===
−
[[https://parts.igem.org/Part:BBa_I13202]]
+
[https://parts.igem.org/Part:BBa_I13202]
−
[[https://parts.igem.org/Part:BBa_T9002]]
+
[https://parts.igem.org/Part:BBa_T9002]

Latest revision as of 01:39, 15 September 2014

quorum sensing machinery that activates GFP expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1815
    Illegal BsaI.rc site found at 2543


Design Notes

Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells.

Source

Combination of two previous parts: BBa_I13202 [1] and BBa_T9002 [2].

References

[1] [2]