Difference between revisions of "Part:BBa K1523101:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | We found that the part BBa_K381001 by iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and nitrite. So we perfomed an experience by this part to see if this part can detect PNP(p-Nitrophenol). | |
+ | We got a good result.You can see the PNP can also induce the green fluorescence. | ||
+ | So we composite the K381001‘s promoter and RBS with a coding sequence of riboflavin(K1172303,iGEM13_Bielefeld-Germany) | ||
===Source=== | ===Source=== |
Latest revision as of 08:05, 7 October 2014
PyeaR->Riboflavin synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1243
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We found that the part BBa_K381001 by iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and nitrite. So we perfomed an experience by this part to see if this part can detect PNP(p-Nitrophenol).
We got a good result.You can see the PNP can also induce the green fluorescence.
So we composite the K381001‘s promoter and RBS with a coding sequence of riboflavin(K1172303,iGEM13_Bielefeld-Germany)
Source
By registry