Difference between revisions of "Part:BBa K1333204:Design"

 
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===Design Notes===
 
===Design Notes===
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This is a composite part including the lambda cI protein and a mutant of LGF2.
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This is a composite part including plac Or2-62 encoded in BBa_K12210 and GFP generator encoded in BBa_E0240. Use a medium RBS and GFP to tell the interactional degree between baits and targets in Bacterial Two-hybrid System.
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===Source===
 
===Source===
  
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Mutated LGF2 at site 78 from Asp to Ala and fused it onto lambda cI.
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BBa_I12210 and BBa_E0240 in stock.
  
 
===References===
 
===References===

Latest revision as of 14:21, 24 September 2014


Or2-RBS-GFP-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


Design Notes

This is a composite part including plac Or2-62 encoded in BBa_K12210 and GFP generator encoded in BBa_E0240. Use a medium RBS and GFP to tell the interactional degree between baits and targets in Bacterial Two-hybrid System.

Source

BBa_I12210 and BBa_E0240 in stock.

References