Difference between revisions of "Part:BBa K1442024:Design"
 
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===Design Notes===
 
===Design Notes===
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None.  
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<p>NKRF IRES nucleotide sequence begins at nucleotide 9 as an AscI and BsrGI restriction site was added in order to allow cloning into the testing module mentioned on the main page. </p>
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<p><b>AscI</b> is compatible with MluI</p>
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<p><b>BsrGI</b> is compatible with BsiWI and TatI</p>
  
 
===Source===
 
===Source===
  
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NF-kappa-B-repressing factor (NKRF) is derived from a human source, the IRES was therefore derived from a human gene.  
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<p>This IRES is derived from the 3' untranslated region of the NKRF mRNA. This a mammalian protein.</p>
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<p>It's efficiency of an IRES was invesigated in the Oumard et al (2000) paper on translationl efficiency 
  
 
===References===
 
===References===
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<b>[1]</b>
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http://iresite.org/IRESite_web.php?page=view&entry_id=140
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<html>
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<body>
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<p></p>
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<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC85491/#!po=12.5000">Oumard et al. 2000</a>
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</body>
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http://www.biotechniques.com/multimedia/archive/00002/BTN_A_000112243_O_2391a.pdf
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</html>
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</p>
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<p><b>[2]</b> <html> <body> <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC55851/#!po=40.7895">Hennecke et al. 2001</a></body> </html> </p>

Latest revision as of 17:08, 7 October 2014

NKRF IRES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


Design Notes

NKRF IRES nucleotide sequence begins at nucleotide 9 as an AscI and BsrGI restriction site was added in order to allow cloning into the testing module mentioned on the main page.

AscI is compatible with MluI

BsrGI is compatible with BsiWI and TatI

Source

This IRES is derived from the 3' untranslated region of the NKRF mRNA. This a mammalian protein.

It's efficiency of an IRES was invesigated in the Oumard et al (2000) paper on translationl efficiency

References

[1] Oumard et al. 2000

[2] Hennecke et al. 2001