Difference between revisions of "Part:BBa K1442028"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1442028 short</partinfo>
 
<partinfo>BBa_K1442028 short</partinfo>
  
−
The HCV NS5B encodes for a RNA Dependent RNA Polymerase (RdRP) that synthesises a complementary strand of RNA when it binds to a RNA sequence.
+
The HCV NS5B encodes for a RNA Dependent RNA Polymerase (RdRP) that synthesises a complementary strand of RNA when it binds to a RNA sequence, this replicon sequence was isolated from HCV 1a and has been tested and confirmed to work in E.coli.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:35, 4 September 2014

HCV NS5B RDRP, (optimised for E.coli)

The HCV NS5B encodes for a RNA Dependent RNA Polymerase (RdRP) that synthesises a complementary strand of RNA when it binds to a RNA sequence, this replicon sequence was isolated from HCV 1a and has been tested and confirmed to work in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 902
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
    Illegal BglII site found at 1340
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 902
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 537