Difference between revisions of "Part:BBa K1346005"
 
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Latest revision as of 18:40, 8 October 2014

pPRM2

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor.

PRM2-zoomed.png

Compared to the other alpha factor responsive promoters, pPRM2 has a low basal expression level, a broad range of expression, and a low maximum output.


140722 AFRP GFP DB ROUND 4 zoomed.png

AFRP GFP DB ROUND 4 140722.png

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]