Difference between revisions of "Part:BBa K1442006:Design"

 
 
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<partinfo>BBa_K1442006 short</partinfo>
 
<partinfo>BBa_K1442006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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None required as was Biobrick compatible  
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<p>This was BioBrick compatible therefore no alterations were required.</p>
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<p>MauBI and MluI sites were added in order to insert the aptazyme into the forwards GFP and EMCV. MauBI and MluI sites are compatible hence CIP must be added in cloning. </p>
  
  
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===Source===
 
===Source===
  
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http://nar.oxfordjournals.org/content/early/2013/04/12/nar.gkt253.full
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<p><b>[1]</b><html><body><a href="http://nar.oxfordjournals.org/content/early/2013/04/12/nar.gkt253.full">Small engineered RNAs</a></body></html></p>
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<p><b>[2]</b><html><body><a href="http://www.readcube.com/articles/10.1093/nar/gkt253">Klauser and Hartig (2012)</a></body></html></p>
  
  
 
===References===
 
===References===

Latest revision as of 21:58, 11 October 2014

Anti-theophylline Aptazyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was BioBrick compatible therefore no alterations were required.

MauBI and MluI sites were added in order to insert the aptazyme into the forwards GFP and EMCV. MauBI and MluI sites are compatible hence CIP must be added in cloning.


Source

[1]Small engineered RNAs

[2]Klauser and Hartig (2012)


References