Difference between revisions of "Part:BBa K1346003"

Latest revision as of 20:45, 3 September 2014

pTEF1(m10) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.

Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units).

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 169

@@ Line 3: / Line 3: @@
 Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.
-**Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
+* Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units).
 [[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
+All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
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