Difference between revisions of "Part:BBa K1346003"

 
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Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.  
 
Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.  
**Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
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* Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units).
  
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
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All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
  
 
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Latest revision as of 20:45, 3 September 2014

pTEF1(m10) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.

  • Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units).

140630 pTEF1 GFP ROUND 1.png

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169