Difference between revisions of "Part:BBa K1491014"
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<partinfo>BBa_K1491014 short</partinfo> | <partinfo>BBa_K1491014 short</partinfo> | ||
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His tagged mRFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | His tagged mRFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | ||
| + | <hr> | ||
| + | <p> | ||
| + | We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). RFP had a high signal-to-noise ratio in both cell lines. RFP was measured at (ex/em = 584nm/607nm). | ||
| + | </p> | ||
| + | https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png | ||
| + | https://static.igem.org/mediawiki/2014/thumb/9/9f/Top10_Best.jpg/591px-Top10_Best.jpg | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:43, 18 October 2014
WTLac-RBS-RFP-His
His tagged mRFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence.
We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). RFP had a high signal-to-noise ratio in both cell lines. RFP was measured at (ex/em = 584nm/607nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]