Difference between revisions of "Part:BBa K1346001:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1346001 short</partinfo>
 
<partinfo>BBa_K1346001 short</partinfo>
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mutant of pTEF1
 
mutant of pTEF1
  
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+
approximately 8 bp of differences.
  
 
===Source===
 
===Source===
  
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endogenous to the s. cerevisiae genome
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Mutant of pTEF1 which is endogenous to the s. cerevisiae genome. Actual mutant found in http://aem.asm.org/content/72/8/5266.long - created by authors.
  
 
===References===
 
===References===
 +
 +
Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae
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Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*
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 +
http://aem.asm.org/content/72/8/5266.long

Latest revision as of 19:09, 15 July 2014

pTEF1(m6) - constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168


Design Notes

mutant of pTEF1

approximately 8 bp of differences.

Source

Mutant of pTEF1 which is endogenous to the s. cerevisiae genome. Actual mutant found in http://aem.asm.org/content/72/8/5266.long - created by authors.

References

Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*

http://aem.asm.org/content/72/8/5266.long