Difference between revisions of "Part:BBa K1346000"

Latest revision as of 20:44, 3 September 2014

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 169

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1346000 short</partinfo>
-Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.
+Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
+[[File:140630 pTEF1 GFP ROUND 1.png|900px]]
+All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
-[[File:140630 pTEF1 GFP ROUND 1.png]]
-<img src="140630 pTEF1 GFP ROUND 1.png" width ="20px"/>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===