Difference between revisions of "Part:BBa K1260002:Design"

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(Design Notes)
 
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===Design Notes===
 
===Design Notes===
The part is a little bit difficult to be PCR because of the same sequence of two linkers on it.
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It has a SH3 domain on the membrane protein LGT's N-terminal so it can combind any protein with SH3 ligand out of the plasma membrane.
 
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===Source===
 
===Source===

Latest revision as of 12:31, 24 May 2014


membrane protein LGT with a SH3 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1084
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 705
    Illegal SapI.rc site found at 202


Design Notes

It has a SH3 domain on the membrane protein LGT's N-terminal so it can combind any protein with SH3 ligand out of the plasma membrane.

Source

It is reconstructed from the BBa_K771007.

References

https://parts.igem.org/Part:BBa_K771007