Difference between revisions of "Part:BBa S03532"
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Construction intermediate | Construction intermediate | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Interestingly, this intermediate conveys Tetracycline resistance to ''E. coli''. Note there is no promoter other than what the vector brings with it. This indicates a "read through" transcription. We are using this intermediate part as a way to screen for ways to insulate the BioBrick parts from "read through" transcription. | + | Interestingly, this intermediate conveys Tetracycline resistance to ''E. coli''. Note there is no promoter other than what the vector brings with it. This indicates a "read through" transcription from pSB1A3. We are using this intermediate part as a way to screen for ways to insulate the BioBrick parts from "read through" transcription. |
Latest revision as of 17:21, 29 October 2006
TetB-RBSrev
Construction intermediate
Usage and Biology
Interestingly, this intermediate conveys Tetracycline resistance to E. coli. Note there is no promoter other than what the vector brings with it. This indicates a "read through" transcription from pSB1A3. We are using this intermediate part as a way to screen for ways to insulate the BioBrick parts from "read through" transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]