Difference between revisions of "Part:BBa J119332"
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<partinfo>BBa_J119332 short</partinfo> | <partinfo>BBa_J119332 short</partinfo> | ||
| − | The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. | + | The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. Mutation at position 430 nt of A to G causes tyrosine (Y) to cysteine (C) in RFP CDS. |
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Latest revision as of 20:07, 9 July 2014
Ammeline Biosensor with Riboswitch M6 + RFP
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. Mutation at position 430 nt of A to G causes tyrosine (Y) to cysteine (C) in RFP CDS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 740
Illegal AgeI site found at 852 - 1000COMPATIBLE WITH RFC[1000]