Difference between revisions of "Part:BBa K823027"
 
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<partinfo>BBa_K823027 short</partinfo>
 
<partinfo>BBa_K823027 short</partinfo>
  
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This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.
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This part is an empty backbone vector for the usage in ''Bacillus subtilis''. It integrates in the ''lacA'' locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in ''E.coli''.
  
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This version still contains NgoMIV restriction sites, therefore is not RFC25-compatible. For an RFC25-compatible version please use the BGSC ECE258 or [https://parts.igem.org/Part:BBa_J179001 BBa_J179001].
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This version contains neither NgoMIV, nor AgeI restriction sites, therefore is RFC25-compatible.  
  
 
<html> <a> <img src="https://static.igem.org/mediawiki/2012/4/46/LMU-Munich-PSBBs2E.png" height=200"/></a></html>
 
<html> <a> <img src="https://static.igem.org/mediawiki/2012/4/46/LMU-Munich-PSBBs2E.png" height=200"/></a></html>
  
  
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This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pAX01.
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This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pAX01. It was also evaluated in the publication [http://www.jbioleng.org/content/7/1/29 The ''Bacillus'' BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with ''Bacillus subtilis''] by Radeck ''et al.''.
  
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]

Latest revision as of 17:02, 3 February 2014

pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.

This version contains neither NgoMIV, nor AgeI restriction sites, therefore is RFC25-compatible.


This backbone is a BioBricked version of the B. subtilis vector pAX01. It was also evaluated in the publication [http://www.jbioleng.org/content/7/1/29 The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis] by Radeck et al..

This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 91
    Illegal NotI site found at 1622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 2591
    Illegal NgoMIV site found at 4919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4743
    Illegal SapI site found at 3660