Difference between revisions of "Part:BBa K1033919"
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<partinfo>BBa_K1033919 short</partinfo> | <partinfo>BBa_K1033919 short</partinfo> | ||
| − | This chromoprotein from the coral ''Galaxea fascicularis'', gfasPurple (also known as gfasCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 577 nm giving it a purple color visible to the naked eye. The | + | This chromoprotein from the coral ''Galaxea fascicularis'', gfasPurple (also known as gfasCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 577 nm giving it a purple color visible to the naked eye. Compared to many other chromoproteins, such as amilCP (<partinfo>BBa_K592009</partinfo>), amilGFP (<partinfo>BBa_K592010</partinfo>), spisPink (<partinfo>BBa_K1033932</partinfo>), asPink (<partinfo>BBa_K1033933</partinfo>) and aeBlue (<partinfo>BBa_K864401</partinfo>), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The protein gfasPurple is closely related to amilCP (<partinfo>BBa_K592009</partinfo>) and has significant sequence homologies with proteins in the GFP family. |
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| + | '''Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: <partinfo>BBa_K1033918</partinfo>.''' | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K1033919 parameters</partinfo> | <partinfo>BBa_K1033919 parameters</partinfo> | ||
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| + | ===Characterization: NWU-China 2019=== | ||
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| + | The NWU-China team overexpressed gfasPurple protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2). | ||
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| + | [[File:T--NWU-China--characterization-BBa_K1033919%28gfasPurple%29.png|900px|thumb|none|alt=fwYellow spectum.|Figure 1. gfasPurple absorbance spectrum]] | ||
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Latest revision as of 13:38, 12 October 2019
gfasPurple, purple chromoprotein
This chromoprotein from the coral Galaxea fascicularis, gfasPurple (also known as gfasCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 577 nm giving it a purple color visible to the naked eye. Compared to many other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The protein gfasPurple is closely related to amilCP (BBa_K592009) and has significant sequence homologies with proteins in the GFP family.
Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: BBa_K1033918.
Usage and Biology
This part is useful as a reporter.
Source
Galaxea fascicularis. The protein was first extracted and characterized by Alieva et. al. under the name gfasCP (GenBank: DQ206394.1). This version is codon optimized for E coli by Genscript.
References
[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, Naila O., et al. "Diversity and evolution of coral fluorescent proteins." PLoS One 3.7 (2008): e2680.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization: NWU-China 2019
The NWU-China team overexpressed gfasPurple protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).
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