Difference between revisions of "Part:BBa K1104101:Design"

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For more information about how we design this part, please move to the biobrick [https://parts.igem.org/Part:BBa_K1104102:Design BBa_K1104102].  <br>
 
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<partinfo>BBa_K1104101 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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whether the RFP will properly express
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After the fusion of both protein, we have to ensure that both protein works well.
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===References===
 
===References===
 
1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism  [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH] <br>
 
1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism  [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH] <br>
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2. Wellens, A., Lahmann, M., Touaibia, M., Vaucher, J., Oscarson, S., Roy, R., Remaut, H., ... Bouckaert, J. (January 01, 2012). The tyrosine gate as a potential entropic lever in the receptor-binding site of the bacterial adhesin FimH. Biochemistry, 51, 24, 4790-9.  <br>

Latest revision as of 19:58, 29 October 2013

For more information about how we design this part, please move to the biobrick BBa_K1104102.

RFP-FimH(mannose-binding domain) fusion protein


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the fusion of both protein, we have to ensure that both protein works well.


Source

Escherichia coli K-12 substrain MG1655 genomic sequence

References

1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH]
2. Wellens, A., Lahmann, M., Touaibia, M., Vaucher, J., Oscarson, S., Roy, R., Remaut, H., ... Bouckaert, J. (January 01, 2012). The tyrosine gate as a potential entropic lever in the receptor-binding site of the bacterial adhesin FimH. Biochemistry, 51, 24, 4790-9.