Difference between revisions of "Part:BBa K1021015"

 
Line 4: Line 4:
 
The geneticin resistance gene (nptII) sits downstream of the constitutive trpC promoter from Aspergillus nidulans. This part can be used as a selectable marker for fungal transformation.
 
The geneticin resistance gene (nptII) sits downstream of the constitutive trpC promoter from Aspergillus nidulans. This part can be used as a selectable marker for fungal transformation.
  
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
 +
*This part was constructed from BBa_K1021007 and BBa_K1021001.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 03:57, 29 October 2013

PtrpC+nptII

The geneticin resistance gene (nptII) sits downstream of the constitutive trpC promoter from Aspergillus nidulans. This part can be used as a selectable marker for fungal transformation.

Usage and Biology

  • This part was constructed from BBa_K1021007 and BBa_K1021001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1004
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 853
    Illegal SapI.rc site found at 1063