Difference between revisions of "Part:BBa K1189034"

 
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<partinfo>BBa_K1189034 short</partinfo>
 
<partinfo>BBa_K1189034 short</partinfo>
 
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This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for RFC 25 standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007).</html>
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This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for <a href="https://parts.igem.org/Help:Standards/Assembly/RFC25">RFC25</a> standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007).</html>
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1189034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1189034 SequenceAndFeatures</partinfo>
  

Latest revision as of 03:18, 29 October 2013

J04500_GFP_freiburg This is a the standard GFP reporter (E0040) that contains the prefix/suffix required for RFC25 standard assembly (K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870