Difference between revisions of "Part:BBa K1189034"
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<partinfo>BBa_K1189034 short</partinfo> | <partinfo>BBa_K1189034 short</partinfo> | ||
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− | This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for | + | This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for <a href="https://parts.igem.org/Help:Standards/Assembly/RFC25">RFC25</a> standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007).</html> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K1189034 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1189034 SequenceAndFeatures</partinfo> | ||
Latest revision as of 03:18, 29 October 2013
J04500_GFP_freiburg This is a the standard GFP reporter (E0040) that contains the prefix/suffix required for RFC25 standard assembly (K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870