Difference between revisions of "Part:BBa K1021023"
(→‎Usage and Biology)
 
(3 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1021023 short</partinfo>
 
<partinfo>BBa_K1021023 short</partinfo>
  
−
The gene for green fluorescent protein sits downstream of the trpC promoter for strong constitutive expression in fungi. This gene can be expressed in Cochliobolus heterostrophus and Ganoderma lucidum.
+
The gene for green fluorescent protein is expressed constitutively under the control of the fungal trpC promoter when transformed into fungi.
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
 +
*Cornell iGEM demonstrated the functionality of this part via transformation (in vector pNG) into ''C. heterosporus.'' The fungal transformants expressed the GFP gene:
 +
https://static.igem.org/mediawiki/2013/6/60/Cochliobolus_fluorescence_composite.png
 +
*The part was constructed from BBa_K1021007 and BBa_E0040.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 03:43, 29 October 2013

PtrpC+GFP

The gene for green fluorescent protein is expressed constitutively under the control of the fungal trpC promoter when transformed into fungi.

Usage and Biology

  • Cornell iGEM demonstrated the functionality of this part via transformation (in vector pNG) into C. heterosporus. The fungal transformants expressed the GFP gene:

Cochliobolus_fluorescence_composite.png

  • The part was constructed from BBa_K1021007 and BBa_E0040.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1019