Difference between revisions of "Part:BBa K1111015"
Sandelisa90 (Talk | contribs) (→Usage and Biology) |
Sandelisa90 (Talk | contribs) (→Usage and Biology) |
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[[File:EPF-Lausanne map.jpg||thumb|400px|center|Figure 1: reminder of BBa_K1111014 expected outcome]] | [[File:EPF-Lausanne map.jpg||thumb|400px|center|Figure 1: reminder of BBa_K1111014 expected outcome]] | ||
| − | + | ==Usage and Biology== | |
| − | This Biobrick is designed to allow the INP to target streptavidin on the outer membrane of E.coli with EYFP as a reporter. | + | This Biobrick is designed to allow the INP to target streptavidin on the outer membrane of E.coli with EYFP between INP and Streptavidin protein as a reporter. |
<br>Indeed EYFP fluorescence will assess of the construct expression and Immunostaining assay (as we did for BBa_K523013) will be used to show the localisation on the cell membrane. | <br>Indeed EYFP fluorescence will assess of the construct expression and Immunostaining assay (as we did for BBa_K523013) will be used to show the localisation on the cell membrane. | ||
<br>Our final goal is to attach biotynilated Gelatine nanoparticules to the E.coli cells thanks to the biotin-streptavidin interaction. | <br>Our final goal is to attach biotynilated Gelatine nanoparticules to the E.coli cells thanks to the biotin-streptavidin interaction. | ||
<br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria. | <br>Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria. | ||
| − | + | ==Sequence and Features== | |
| − | + | ||
<partinfo>BBa_K1111015 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1111015 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:28, 28 October 2013
INP_EYFP_Streptavidin Fusion Protein
Introduction
This part is a Fusion protein made respectively of an Ice Nucleation Protein (INP), EYFP and a Streptavidin protein; which is an improved version of BBa_K1111014. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.
Usage and Biology
This Biobrick is designed to allow the INP to target streptavidin on the outer membrane of E.coli with EYFP between INP and Streptavidin protein as a reporter.
Indeed EYFP fluorescence will assess of the construct expression and Immunostaining assay (as we did for BBa_K523013) will be used to show the localisation on the cell membrane.
Our final goal is to attach biotynilated Gelatine nanoparticules to the E.coli cells thanks to the biotin-streptavidin interaction.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2384
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
Illegal AgeI site found at 2426
Illegal AgeI site found at 2477 - 1000COMPATIBLE WITH RFC[1000]