Difference between revisions of "Part:BBa K1088000"
 
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From Biocyc:
 
From Biocyc:
 
"Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways."
 
"Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways."
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http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER Source.]
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[http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER Source.]
  
  

Latest revision as of 11:29, 28 October 2013

1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168

The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao L et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs) gene as well as the enzyme catalyzing the second step of the MEP pathway. They observed a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).

Source: Lishan Zhao, Wei-chen Chang, Youli Xiao, Hung-wen Liu and Pinghua Liu: Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis, Annu. Rev. Biochem. 2013. 82:497-530, page 500. [http://www.ncbi.nlm.nih.gov/pubmed/23746261 (link)]

From Biocyc: "Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways." [http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER Source.]



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
    Illegal NgoMIV site found at 1020
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 862
    Illegal SapI.rc site found at 1561