Difference between revisions of "Part:BBa J31007:Design"

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===Design Notes===
 
===Design Notes===
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It was cloned into pSB1A2 plamsid.
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This sequence was amplified from pSB1A3 and is identical to the TetR gene found in pBR322.
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This sequence was amplified from <partinfo>pSB1AT3</partinfo> and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.
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It turns out, this tetR gene actually has two open reading frames encoding two different proteins.
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This part was PCR amplified from <partinfo>pSB1AT3</partinfo> using the following primers. The primers have non-annealing 5'- extensions that introduce an <font color='red'>XbaI site</font> to the left and a <font color='blue'>SpeI site</font> to the right of the coding region. Primer annealing sites are shown in bold.
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<br>Forward: 5’ GCAT<font color='red'>TCTAG <b>A</b></font><b>TGAAATCTAACAATGCGCTCATC</b>
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<br>Reverse: 5’ ATGC<font color='blue'>ACTAG <b>T</b></font><b>TAGGTCGAGGTGGCCCGGC</b>
  
 
===Source===
 
===Source===
  
−
PsB1AT3
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<partinfo>pSB1AT3</partinfo>
  
 
===References===
 
===References===

Latest revision as of 21:52, 6 December 2006


tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.

This part was PCR amplified from pSB1AT3 using the following primers. The primers have non-annealing 5'- extensions that introduce an XbaI site to the left and a SpeI site to the right of the coding region. Primer annealing sites are shown in bold.
Forward: 5’ GCATTCTAG ATGAAATCTAACAATGCGCTCATC
Reverse: 5’ ATGCACTAG TTAGGTCGAGGTGGCCCGGC

Source

pSB1AT3

References