Difference between revisions of "Part:BBa M36661:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_M36661=== | ===Applications of BBa_M36661=== | ||
| + | <nowiki>Insert non-form[[File:Example.jpg]]atted text here</nowiki> | ||
| + | |||
| + | ===Stanford Location=== | ||
| + | Plasmid Name: fur protein, | ||
| + | DNA 2.0 Gene #: 135239, | ||
| + | Organism: K12 E.coli, | ||
| + | Device Type: Sensor, | ||
| + | Box Label: BIOE 44 F13, | ||
| + | Barcode #'s of Glycerol Stocks: | ||
| + | 0133011789, | ||
| + | 0113010769, | ||
| + | 0133009689, | ||
| + | 0133011828, | ||
| + | 0133009694, | ||
| + | 0133011775(This one used to Restart Stock for Fur Sensor Project) | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_M36661 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_M36661 StartReviews</partinfo> | ||
| − | + | Results of Beta Gal Tests | |
| − | + | ||
| − | + | ANOVA of BETA GAL Round 1—p=.232 NOT SIGNIFICANT | |
| − | + | ||
| − | + | ANOVA of BETA GAL Round 2—p=.389 NOT SIGNIFICANT | |
| − | + | ||
| − | + | Student t-test Beta Gal round 2 | |
| − | + | ||
| − | + | -4 vs -7: | |
| − | + | t= -1.43 | |
| + | sdev= 6.85 | ||
| + | degrees of freedom = 14 The probability of this result, assuming the null hypothesis, is 0.18(Not significant) | ||
| + | |||
| + | -4 vs no iron: | ||
| + | t= -1.80 | ||
| + | sdev= 9.15 | ||
| + | degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.097(Not significant) | ||
| + | |||
| + | -7 vs no iron: | ||
| + | t=-0.823 | ||
| + | sdev= 9.03 | ||
| + | degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.43(Not significant) | ||
| + | |||
| + | [[Image:betagal1.png]] | ||
| + | |||
| + | Figure #1 (above):Relationship of the Beta-Gal production from the second test. Each category contained n=4 duplicates. Values were averaged and standard deviations were calculated to develop the above graph. Analysis with One-Way ANOVA (p=.389) and Student t-tests between groups showed no statistical significance. | ||
| + | |||
| + | The results from this first round showed a promising difference between the iron concentration of 10^-4 M and no iron, as we expected. This led us to develop a second round of experimentation for look for statistical differences with more samples. Since there was not a significant difference between the groups, we also steered away from looking for a gradient and spent our efforts testing for functionality in the second round of experimentation. | ||
| + | |||
| + | [[Image:betagal2.png]] | ||
| + | |||
| + | Figure #2 (above):Relationship of the Beta-Gal production from the first round of experimentation. Each category contained n=2 duplicates. Values were averaged and standard deviations were calculated to develop the above graph. Analysis with One-Way ANOVA (p=.232) showed no statistical significance. (NOTE: There were too few duplicates per category to perform Student t-tests between groups.) | ||
| + | |||
| + | Our second round of experimentation did not yield a significant difference between groups, suggesting that our construct is not functional. | ||
| + | |||
| + | [[Image:pcr2.png]] | ||
| + | |||
| + | Figure #3: (above) The results from Gel electrophoresis demonstrated successful transcription (shown by the bright bands). The results also suggest the presence of our DNA (shown by the lighter bands). Bright bands present at: 8 (RT-PCR) and 9 (PCR, plasmid DNA). Light bands present at: 2 (PCR negative control),6 (RT-PCR), 10 (PCR- same DNA as 3) | ||
| + | |||
| + | [[Image:plate1.png]] | ||
| + | |||
| + | Figure #4: (above) Very faint fluorescence shows some potential expression. | ||
| + | |||
| + | |||
| + | [[Image:plate2.png]] | ||
| + | |||
| + | Figure #5: (above) Amp plate—plasmid was taken up. | ||
| + | |||
| + | [[Image:plate3.png]] | ||
| + | |||
| + | Figure #6: (above) Kan plate—no growth as expected | ||
<!-- DON'T DELETE --><partinfo>BBa_M36661 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_M36661 EndReviews</partinfo> | ||
Latest revision as of 22:02, 6 December 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36661
Insert non-form[[File:Example.jpg]]atted text here
Stanford Location
Plasmid Name: fur protein, DNA 2.0 Gene #: 135239, Organism: K12 E.coli, Device Type: Sensor, Box Label: BIOE 44 F13, Barcode #'s of Glycerol Stocks: 0133011789, 0113010769, 0133009689, 0133011828, 0133009694, 0133011775(This one used to Restart Stock for Fur Sensor Project)
User Reviews
UNIQ66609941c569c1a7-partinfo-00000001-QINU Results of Beta Gal Tests
ANOVA of BETA GAL Round 1—p=.232 NOT SIGNIFICANT
ANOVA of BETA GAL Round 2—p=.389 NOT SIGNIFICANT
Student t-test Beta Gal round 2
-4 vs -7: t= -1.43 sdev= 6.85 degrees of freedom = 14 The probability of this result, assuming the null hypothesis, is 0.18(Not significant)
-4 vs no iron: t= -1.80 sdev= 9.15 degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.097(Not significant)
-7 vs no iron: t=-0.823 sdev= 9.03 degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.43(Not significant)
Figure #1 (above):Relationship of the Beta-Gal production from the second test. Each category contained n=4 duplicates. Values were averaged and standard deviations were calculated to develop the above graph. Analysis with One-Way ANOVA (p=.389) and Student t-tests between groups showed no statistical significance.
The results from this first round showed a promising difference between the iron concentration of 10^-4 M and no iron, as we expected. This led us to develop a second round of experimentation for look for statistical differences with more samples. Since there was not a significant difference between the groups, we also steered away from looking for a gradient and spent our efforts testing for functionality in the second round of experimentation.
Figure #2 (above):Relationship of the Beta-Gal production from the first round of experimentation. Each category contained n=2 duplicates. Values were averaged and standard deviations were calculated to develop the above graph. Analysis with One-Way ANOVA (p=.232) showed no statistical significance. (NOTE: There were too few duplicates per category to perform Student t-tests between groups.)
Our second round of experimentation did not yield a significant difference between groups, suggesting that our construct is not functional.
Figure #3: (above) The results from Gel electrophoresis demonstrated successful transcription (shown by the bright bands). The results also suggest the presence of our DNA (shown by the lighter bands). Bright bands present at: 8 (RT-PCR) and 9 (PCR, plasmid DNA). Light bands present at: 2 (PCR negative control),6 (RT-PCR), 10 (PCR- same DNA as 3)
Figure #4: (above) Very faint fluorescence shows some potential expression.
Figure #5: (above) Amp plate—plasmid was taken up.
Figure #6: (above) Kan plate—no growth as expected UNIQ66609941c569c1a7-partinfo-00000002-QINU