Difference between revisions of "Part:BBa K1093003"

Latest revision as of 18:47, 12 October 2013

sfBFP N-terminal

Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to BFP it displays improved stability and stronger blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm. This is the N-terminus of sfBFP (split between amino acids 214/215), that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific). The 'wild type' C-terminus is not applicable, as it spontaneously recreates the native fluorophore with the N-terminal part (although it can be used as an alternative for classical alpha complementation). The part contains Freiburg prefix (suitable for fusion at the N-terminus).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub 2011 Jan 27. PubMed PMID: 21273204.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1093003 short</partinfo>
-Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm.
+Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to BFP it displays improved stability and stronger blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm.
-This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific).
+This is the N-terminus of sfBFP (split between amino acids 214/215), that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific). The 'wild type' C-terminus is not applicable, as it spontaneously recreates the native fluorophore with the N-terminal part (although it can be used as an alternative for classical alpha complementation). The part contains Freiburg prefix (suitable for fusion at the N-terminus).
 ==References==
@@ Line 10: / Line 10: @@
 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
 Sep;24(9):1170. PubMed PMID: 16369541.
 : Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence
 complementation tool based on superfolder green fluorescent protein. Acta Biochim