Difference between revisions of "Part:BBa K1055001:Experience"
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===Applications of BBa_K1055001=== | ===Applications of BBa_K1055001=== | ||
− | When tried to fuse to the Tar receptor of ''E. coli'' C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct. Please take a look below: | + | When tried to fuse to the Tar receptor of ''E. coli'' C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 318 kj/mol). Please take a look below: |
− | [[Image:mfold_Lssmorange.png| | + | [[Image:mfold_Lssmorange.png|center|400px|thumb|Hypothetical DNA secondary structure of Tar-LssmOrange fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 318 kj/mol]] |
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===User Reviews=== | ===User Reviews=== |
Latest revision as of 18:09, 12 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1055001
When tried to fuse to the Tar receptor of E. coli C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 318 kj/mol). Please take a look below:
User Reviews
UNIQdfa4b1012e597e5c-partinfo-00000000-QINU UNIQdfa4b1012e597e5c-partinfo-00000001-QINU