Difference between revisions of "Part:BBa J23058"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_J23058 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J23058 SequenceAndFeatures</partinfo>
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gcgggcgggcgggcgggcgggatccataaaaaaaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaaaaaaagcttcccgcccgcccgcccgcccgt
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Latest revision as of 03:15, 11 August 2006

[GFPmut3] inserted into [key3d]

This is an intermediate that aids in cloning via introducing a phenotype that allows for easy distinction between the parent and clone. The goal is build a construct similiar to J23009 with a longer hairpin loop (10 bases total). By inserting this in the BamHI and HindIII sites the successful subclones should be green (hence GFP), parent will be white. Conversely, when cutting out the GFPmut3 cassette and inserting the longer hairpin the successful clones will be white and the parent will still be green. This makes screening colonies much easier.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 759
    Illegal BamHI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 685