Difference between revisions of "Part:BBa K1223003"

 
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kanamicin ressistance gene (promoter+CDS)
 
kanamicin ressistance gene (promoter+CDS)
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===Design Notes===
 
===Design Notes===
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 12:25, 12 October 2013

Kanamicin ressistance

kanamicin ressistance gene (promoter+CDS)


Design Notes

selection gene for that confers the resistance to Kanamycin/Neomycin antibiotics. part is a synthetic construct also used in the registry's pSB3K5 plasmid backbone.

this part was used as a selection gene for the P.A.S.E. 1 and 2 cassettes.

this part contains the promoter, CDS and stop codon.

Source

part was extracted from the pKD4 plasmid and is similar to entry EU496093 on Genbank.

References

Shetty, R.; Endy, D.; Knight, T. Journal of Biological Engineering 2008, 2, 5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]