Difference between revisions of "Part:BBa K1144015"
 
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RcnR is a protein that constantly represses PrcnA. When there is nickel in the media RcnR alters its form, making it impossible to bind PrcnA, activating the production of the HoxN protein. This last protein is an efficient nickel transporter from ''Cupriavidus metallidurans'' that places in the membrane allowing the metal to get into the cell.
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Prcnr,rcnR and PrcnA genes were used from E.coli regulation system.
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Prcnr is a constitutive promoter, controlling the production of RcnR protein which
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represses PrcnA. When there is nickel in the medium RcnR this induces a conformational
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change in RcnR which prevents its binding to PrcnA, activating the production of the HoxN  
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protein in our construct. HoxN an efficient nickel membrane transporter form Cupriavidus  
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metallidurans that imports the metal into the cell. RFP was transcriptionally fused to HoxN
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to monitor its expression.
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Therefore, our system continuously detecting for nickel, and when this metal is detected,
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it is absorbed.
  
 
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===References===
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Eitinger, T. Microbial Nickel Transport In Microbial Transport Systems. Ed. G. Winkelmann. Wiley-VCH 2001

Latest revision as of 22:11, 5 October 2013

Prcna, hoxN


Prcnr,rcnR and PrcnA genes were used from E.coli regulation system.

Prcnr is a constitutive promoter, controlling the production of RcnR protein which

represses PrcnA. When there is nickel in the medium RcnR this induces a conformational

change in RcnR which prevents its binding to PrcnA, activating the production of the HoxN

protein in our construct. HoxN an efficient nickel membrane transporter form Cupriavidus

metallidurans that imports the metal into the cell. RFP was transcriptionally fused to HoxN

to monitor its expression.

Therefore, our system continuously detecting for nickel, and when this metal is detected,

it is absorbed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 945
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Eitinger, T. Microbial Nickel Transport In Microbial Transport Systems. Ed. G. Winkelmann. Wiley-VCH 2001